Model systems drive biological research by recapitulating body processes and functions from the molecular to whole organism level. The human body is composed of both cellular and non-cellular material organized in a specialized manner. It is difficult to mimic all aspects of human biology with one in-vitro model. 3D cell culture models are a more accurate representation of the natural environment experienced by cells in the living organism as opposed to growing cells on 2D flat surfaces. In particular, organoids are in-vitro derived 3D cell aggregates derived from primary tissue or stem cells that are capable of self-renewal, self-organization and exhibit organ functionality. Organoids are physiologically relevant and amenable to molecular and cell biological analyses, holding great promise in both basic research and translational applications. The workshop is focused on the identification of organoid models of human diseases, as cancer and cystic fibrosis. The history of organoids and their future applications in the biomedical research is also discussed.